The rectangular panel of the sacrifice of Isaac is situated on the northern side of the nave below the Zodiac panel and is the first panel which visitors see upon entry. On the right of the panel, Abraham and Isaac are depicted before an altar. A ram is set in the middle of the panel beneath a hand in the sky above, and two servants stand with an ass on the left. Above this scene, is a row of palm trees set in a band separated from the main panel by a black line.
Abraham, a tall bearded figure, wears a long tunic and has a halo around his head. He stands before a rectangular burning altar and holds up young Isaac in his left hand, towards the altar. Abraham holds a long knife in his right hand before him. Isaac, depicted as a small child, wears a scarf on his neck and crosses his arms. Hebrew inscriptions of their names are situated above these figures.
The standing ram, which is overturned and set vertically on its tail, is positioned to the right of Abraham, tied by a rope to a tree. Inscribed in Hebrew above the ram, are the words: "And behold.... a ram" (Gen. 22:13).
Above the ram, the hand of God emerges towards Abraham from a cloud with rays. The cloud overlaps the band of palm trees and the main panel. Below the hand is a Hebrew inscription which reads: “Lay not thine" (Gen. 22:12).
The two servants and the ass stand on the left by the ram. The servant on the right wears a short tunic and holds the reins of the ass in one hand and a whip in the other. The servant on the left stands behind the ass. The ass wears a bell around its neck and has a saddle on its back.
Branches are scattered beneath the servants and the ram, and to the right of Abraham.
The outlines of figures and other elements consist of one or two lines of black tesserae, except for the figures of Isaac and the ram which are outlined in red and ochre.
The colors of the tesserae, are white and black and shades of: gray, brick red, wine red and light brown. shades of grey, brick red, wine red and light brown.